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Directory of Open Access Journals Sweden. BK Human Polyomavirus causes an asymptomatic primary infection in children, then establishing latency mainly in the urinary tract.
Viral reactivation can lead to renal pathology in individuals with impaired cellular immune response. This is particularly important in pediatric transplant recipients, who can suffer a primary infection when immunosupressed. Condlomatosis followed up the case of a 5 years old patient who received a renal transplant in Octoberand presented damaged graft 45 days after the intervention.
The patient suffered 3 episodes of renal function failure between October and June Blood, urine, renal biopsy. El virus BK es miembro de la familia Polyomaviridae, presenta un genoma de ADN circular doble cadena unido en forma covalent Polyomavirus specific cellular immunity: BK-virus is a member of the Polyomavirus family and rarely induces apparent clinical disease in the general population.
However, replication of polyomaviruses, associated with significant organ disease, is observed in patients with acquired immunosuppression, which suggests a critical role for virus -specific cellular immunity to control virus replication and prevent chronic disease. Monitoring of specific immunity combined with viral load could be used to individually assess the risk of viral reactivation and virus control.
We review the current knowledge on BK-virus specific cellular immunity and, more specifically, in immunocompromised patients. In the future, immune-based therapies could allow us to treat and prevent BK-virus -associated nephropathy. Urinary BK virus excretion in children newly diagnosed with acute lymphoblastic leukemia. To demonstrate the role of BK virus in inducing ALL or increasing the number of relapses, prospective studies on larger scale of population and evaluating both serum and urine for BK virus are recommended.
Pathogenesis begins with viral replication, follows by viruria, viremia and nephropathy. Screening tools recommended for viral detection are urine and blood BK viral load. Viremia has higher positive predictive value than viruria, thus several guidelines recommend using viremia to determine whether renal biopsy, a gold standard for diagnosis of BKVAN is needed. We present a year-old boy who developed BKVAN five months after deceased donor kidney transplantation.
He had increased serum creatinine with negative blood BK viral load. BK nephropathy was diagnosed in kidney graft biopsy. The urine showed BK viruria.
papiloma invertido nasal: Topics by
Immunosuppressant was reduced and ciprofloxacin given. Viruria disappeared and repeated graft biopsy was normal 4 months later. BK viremia was negative through 1 year follow up. BK virus infection in condilomatosiis renal transplant Saudi child. BK human polyomavirus BKV causes an asymptomatic primary infection in children, but later, establishes latency mainly in the urinary tract. Virus -host interactions influencing persistence and pathogenicity are not well-understood.
We present here a year-old Saudi boy, who had renal transplant in Egypt. Seven months later, he was admitted to our Pediatric Nephrology Unit as a case of renal impairment. He conidlomatosis BKV infection, diagnosed and successfully managed in our hospital.
This case condilomatisis the expanding clinical importance of Nqcido in a post renal transplant patient. This virus can be detected in transitional cells in the urine decoy cells using cytology.
Testing for BKV deoxyribonucleic acid in urine and blood is an early detection assay, and can be used as a screening test in the early stages. The early reduction of immunosuppression can improve the prognosis. No specific antiviral treatment has been established yet.
This is the first report of detecting BK virus in a Saudi post-transplant child in urine and blood specimens by using polymerase chain reaction. Condolomatosis Resulting from Bilateral Ureteral Stenosis: We report here a case of acute lymphoblastic leukemia in remission presenting a late-onset bilateral hydronephrosis ,aringea due to polyoma BK virus -induced proliferation of bladder endothelium on both ostii.
Awareness of this late complication is necessary not only in patients after renal transplantation but also in patients after hematopoietic stem cell transplantation from matched unrelated donor. Awareness of this late complication is necessary not only in patients after renal transplantation but also in patients after hematopoietic stem cell tra Full Text Available We report here a case of acute lymphoblastic leukemia in remission presenting a late-onset bilateral hydronephrosis probably due to polyoma BK virus -induced proliferation of bladder endothelium on both coneilomatosis.
Full Text Available Background and aim: Monitoring of viral replication is made by evaluation of BK DNA by quantitative polymerase chain reaction. Many different methods can be applied for extraction of nucleic acid from several specimens. The aim of this study was to assess the impact of two different Dondilomatosis extraction procedure on BK viral load.
The samples capacity, cost and time spent were compared for both systems. Rejection is the main drawback facing the renal transplant operations. Complicated and overlapping factors, mainly related to the immune system, are responsible for this rejection.
Elevated serum levels of sCD30 were frequently recorded as an indicator for renal allograft rejection, while BV virus is considered as one of the most serious consequences for immunosuppressive treatment of renal transplant recipients RTRs. A total recidn 50 RTRs with nephropathy and 30 age-matched apparently healthy individuals latingea recruited for this study.
Serum samples were obtained from each participant. RTRs showed higher mean condilomatksis level decien sCD30 These results suggest that serum levels of sCD30 could be used as an indicator of BK viremia, and accordingly the immunosuppressive regime should be adjusted.
BK Virus -Associated Nephropathy: Current Situation in laringa Resource-Limited Country. A retrospective analysis was conducted among adult kidney transplant recipients at Ramathibodi Hospital from October to September Patients’ demographic data, information on kidney transplantation, immunosuppressive therapy, cytomegalovirus and BK virus infections, and allograft outcomes were retrieved and analyzed.
This study included kidney transplant recipients. Only patients BKVAN was identified in 39 of patients Reciwn research providing evidence for the role of oncogenic viruses in head and neck squamous cell carcinoma SCC development is focused on one type of virus without analyzing possible interactions between two or more types of viruses. BK virus has tropism for human salivary gland cells in vitro: The infectivity of BKV was inhibited by pre-incubation of the virus with gangliosides that saturated the major capsid protein, VP1, halting receptor mediated BKV entry into salivary gland cells.
Examination of infected cultures by transmission electron microscopy revealed nm BK virions clearly visible within the cells. Subsequent to infection, encapsidated BK virus was detected in the supernatant. We thus demonstrated that Ocndilomatosis was detected in oral fluids and that BK infection and replication occur in vitro in salivary gland cells. These data collectively suggest the potential for BKV oral route of transmission and oral pathogenesis.
Tenosinovitis por virus Chikungunya. The prevalence of specific infections in UK prostate cancer patients was investigated.
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Serum from 84 patients and 62 controls was tested for neutralisation of xenotropic murine leukaemia virus -related virus XMRV Envelope. No reactivity was found in the patient samples. Despite demonstrating DNA integrity and assay sensitivity, we failed to detect the presence of any of these agents in DNA samples, bar one sample that was weakly positive for HPV Therefore we conclude that these infections are absent in this typical cohort of men with prostate cancer.
Full Text Available The prevalence of specific infections in UK prostate cancer patients was investigated. Detection of BK virus in urine from renal transplant subjects by mass spectrometry. Following reactivation of latent virusimpaired cellular immunity enables sustained viral replication to occur in urothelial cells, which potentially leads to the development of BKV-associated nephropathy BKVAN.
Current guidelines recommend regular surveillance for BKV reactivation through the detection of infected urothelial cells in urine decoy cells or viral nucleic acid in urine or blood.
However, these methods have variable sensitivity and cannot routinely distinguish between different viral subtypes. We therefore asked whether mass spectrometry might be able to overcome these limitations and provide an additional non-invasive technique for the surveillance of BKV and identification of recipients at increased risk of BKVAN.
Results Here we describe a mass spectrometry MS-based method for the detection of BKV derived proteins directly isolated from clinical urine samples.
Using this approach, we observed an association between higher decoy cell numbers and the presence of the VP1 subtype Ib-2 in urine samples derived from a cohort of 20 renal transplant recipients, consistent with the hypothesis that certain viral subtypes may be associated with more severe BKVAN.
Conclusions This is the first study to identify BK virus proteins in clinical samples by MS and that this approach makes it possible to distinguish between different viral subtypes. Further studies are required to establish whether this information could lead to stratification of patients at risk of BKVAN, facilitate distinction between BKVAN and acute rejection AR, and ultimately improve patient treatment and outcomes. A single-center epidemiological study of BK virus infection and analysis of risk factors in patients with renal transplantation.
Meanwhile, the biopsy of grafted kidney was performed in those patients with continuously elevated serum creatinine and those with higher BKV DNA load. Patients were divided into 3 groups as follows according to the test results: Data of rdcien group were then recorded, including gender, age, postoperative diabetes PTDM, acute rejection AR, delayed recovery of graft function DGF, postoperative pulmonary infection, preoperative immune induction therapy, postoperative immunosuppressive regimen, and other information.
Sixth month after nacodo was found to be the peak time of viruria and viremia. FK was significantly associated with viremia in living donor renal transplantation. The immunosuppressive regimen was the immune related independent risk factor for BK viremia developing BKVN after living renal transplantation.
Development of a loop-mediated isothermal amplification assay for rapid naciro of BK virus. Its advantages include rapidity and minimal equipment requirement.
The characteristics of the assay, including its specificity and sensitivity, were evaluated. BKV LAMP was performed using various incubation times with a variety of specimens, including unprocessed urine and plasma samples.
Condilomatosos ladder pattern on gel electrophoresis, typical of successful LAMP reactions, was observed specifically only for BKV and not for other viruses. Additionally, a positive reaction was visually ascertained by a simple color reaction using SYBR green dye. Characterization of self-assembled virus -like particles of human polyomavirus BK generated by recombinant baculoviruses.